Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea).

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080924" target="_blank" >RIV/00216224:14310/15:00080924 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea).

Popis výsledku v původním jazyce

Our research is focused primarily on expression and characterization of cystatin produced by platyhelminth Eudiplozoon nipponicum. This blood-feeding ectoparasite of Cyprinus carpio (common carp) is a representative of the family Diplozooidae (Monogenea). Cystatins ? cysteine peptidase inhibitors are produced by a wide range of organisms and belong to bioactive molecules with immunomodulatory and inhibitory properties. The transcriptomic data of E. nipponicum were analyzed for the presence of cystatin sequences. The partial nucleotide sequence was identified and the whole gene sequence was obtained by PCR, 3?/5? RACE PCR and sequencing using cDNA as a template. Gene coding 98 amino acid cystatin of E. nipponicum with predicted molecular weight 10.85 kDa and theoretical pI 6.27 was inserted into pET19b expression vector and the obtained construct was transferred into E. coli competent cells (BL 21 strain). Expression of recombinant cystatin molecule was induced by adding IPTG to the cul

Název v anglickém jazyce

Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea).

Popis výsledku anglicky

Our research is focused primarily on expression and characterization of cystatin produced by platyhelminth Eudiplozoon nipponicum. This blood-feeding ectoparasite of Cyprinus carpio (common carp) is a representative of the family Diplozooidae (Monogenea). Cystatins ? cysteine peptidase inhibitors are produced by a wide range of organisms and belong to bioactive molecules with immunomodulatory and inhibitory properties. The transcriptomic data of E. nipponicum were analyzed for the presence of cystatin sequences. The partial nucleotide sequence was identified and the whole gene sequence was obtained by PCR, 3?/5? RACE PCR and sequencing using cDNA as a template. Gene coding 98 amino acid cystatin of E. nipponicum with predicted molecular weight 10.85 kDa and theoretical pI 6.27 was inserted into pET19b expression vector and the obtained construct was transferred into E. coli competent cells (BL 21 strain). Expression of recombinant cystatin molecule was induced by adding IPTG to the cul

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů