In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087850" target="_blank" >RIV/00216224:14310/16:00087850 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00436-015-4841-0" target="_blank" >http://dx.doi.org/10.1007/s00436-015-4841-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-015-4841-0" target="_blank" >10.1007/s00436-015-4841-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Popis výsledku v původním jazyce

This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C.

Název v anglickém jazyce

In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Popis výsledku anglicky

This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology Research

ISSN

0932-0113

e-ISSN

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Svazek periodika

115

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1113-1121

Kód UT WoS článku

000370868200021

EID výsledku v databázi Scopus

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