Statistical comparison of excystation methods in Cryptosporidium parvum oocysts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00468718" target="_blank" >RIV/60077344:_____/16:00468718 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/16:00088266 RIV/60076658:12220/16:43891334

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetpar.2016.10.007" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2016.10.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2016.10.007" target="_blank" >10.1016/j.vetpar.2016.10.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Statistical comparison of excystation methods in Cryptosporidium parvum oocysts

Popis výsledku v původním jazyce

Excystation of sporozoites of Cryptosporidium parvum from oocysts is essential for successful in vitro assays. It has also been traditionally used as a measure for oocyst viability and infectivity. Laboratories use various excystation protocols so there is a need to clarify which method is the best. In this study, six different protocols for in vitro excystation of C. parvum oocysts were compared to find the most efficient excystation method (expressed as percentage excystation). Tested protocols differed in chemical pre-incubation steps, excystation media or time of incubation.nThere were significant differences in percentage of excysted oocysts among groups excysted by different methods. There were also significant differences in percentage of excysted oocysts between methods using pre-incubation with sodium hypochlorite and those without. The other variables examined; the presence of trypsin, kind of excystation medium and the incubation time, did not show statistical differences in percentage excystation among groups.nPre-incubation steps which included sodium hypochlorite, enhancing the permeability of the oocysts were found to increase the excystation ratio and methods using this step were the most effective.

Název v anglickém jazyce

Statistical comparison of excystation methods in Cryptosporidium parvum oocysts

Popis výsledku anglicky

Excystation of sporozoites of Cryptosporidium parvum from oocysts is essential for successful in vitro assays. It has also been traditionally used as a measure for oocyst viability and infectivity. Laboratories use various excystation protocols so there is a need to clarify which method is the best. In this study, six different protocols for in vitro excystation of C. parvum oocysts were compared to find the most efficient excystation method (expressed as percentage excystation). Tested protocols differed in chemical pre-incubation steps, excystation media or time of incubation.nThere were significant differences in percentage of excysted oocysts among groups excysted by different methods. There were also significant differences in percentage of excysted oocysts between methods using pre-incubation with sodium hypochlorite and those without. The other variables examined; the presence of trypsin, kind of excystation medium and the incubation time, did not show statistical differences in percentage excystation among groups.nPre-incubation steps which included sodium hypochlorite, enhancing the permeability of the oocysts were found to increase the excystation ratio and methods using this step were the most effective.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP505%2F11%2F1163" target="_blank" >GAP505/11/1163: Protizánětlivá aktivita extraktů z rostlin Indonésie a jejich účinek na průběh oportunních parazitóz</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

—

Svazek periodika

230

Číslo periodika v rámci svazku

OCT 30

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

000389733000001

EID výsledku v databázi Scopus

2-s2.0-85006736242