MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087859" target="_blank" >RIV/00216224:14310/16:00087859 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/16:00066191

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201500587" target="_blank" >http://dx.doi.org/10.1002/elps.201500587</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201500587" target="_blank" >10.1002/elps.201500587</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

Popis výsledku v původním jazyce

Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo’s physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo’s surroundings can determine the embryo’s physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo’s surroundings was developed. AAs were analyzed by means of MEKC-LIF after on capillary derivatization by naphthalene 2,3 dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology.

Název v anglickém jazyce

MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

Popis výsledku anglicky

Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo’s physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo’s surroundings can determine the embryo’s physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo’s surroundings was developed. AAs were analyzed by means of MEKC-LIF after on capillary derivatization by naphthalene 2,3 dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F11%2F0009" target="_blank" >GAP206/11/0009: Kapilární electroforéza jako nástroj pro metabolomické studie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

17-18

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

2305-2312

Kód UT WoS článku

000383596800004

EID výsledku v databázi Scopus

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