Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088273" target="_blank" >RIV/00216224:14310/16:00088273 - isvavai.cz</a>

Výsledek na webu

<a href="http://femsle.oxfordjournals.org/content/363/19/fnw211" target="_blank" >http://femsle.oxfordjournals.org/content/363/19/fnw211</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnw211" target="_blank" >10.1093/femsle/fnw211</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage

Popis výsledku v původním jazyce

The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages phi29, phi52A and phi80alpha as well as by prophage phi53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains.

Název v anglickém jazyce

Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage

Popis výsledku anglicky

The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages phi29, phi52A and phi80alpha as well as by prophage phi53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-05069P" target="_blank" >GP13-05069P: Horizontální přenos stafylokokové chromozomové kazety mec (SCCmec) prostřednictvím bakteriofágů napříč kmeny rodu Staphylococcus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Svazek periodika

363

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

"nestrankovano"

Kód UT WoS článku

000388381800005

EID výsledku v databázi Scopus

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