Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057819" target="_blank" >RIV/00216224:14310/12:00057819 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x" target="_blank" >http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x" target="_blank" >10.1111/j.1574-6968.2012.02589.x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
Popis výsledku v původním jazyce
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone.
Název v anglickém jazyce
Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
Popis výsledku anglicky
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA310%2F09%2F0459" target="_blank" >GA310/09/0459: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
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Svazek periodika
332
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
7
Strana od-do
146-152
Kód UT WoS článku
000305334300008
EID výsledku v databázi Scopus
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