Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057818" target="_blank" >RIV/00216224:14310/12:00057818 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/75010330:_____/12:00009830

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs00203-012-0797-y" target="_blank" >http://link.springer.com/article/10.1007%2Fs00203-012-0797-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00203-012-0797-y" target="_blank" >10.1007/s00203-012-0797-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains

Popis výsledku v původním jazyce

The aim of this study was to compare the plasmid contents of methicillin-resistant Staphylococcus aureus (MRSA) strains classified into different clonal clusters (CCs). The isolates were collected from 15 Czech hospitals in 2000?2008. Plasmid DNA was detected in 65 (89%) strains and 33 of them harbored more than one plasmid type. Altogether 24 different types of plasmids were identified, ranging in size from 1.3 to 55 kb. Restriction endonuclease analysis, plasmid elimination, DNA hybridization, and sequencing were used for their further characterization. It has been found that the conjugative, erythromycin resistance and enterotoxin D encoding plasmids are harbored by strains from different CCs. On the other hand, chloramphenicol and tetracycline resistance plasmids, and most of the penicillinase and cryptic plasmids were only detected in certain CCs. Especially the pUSA300-like plasmids were found exclusively in the USA300 clone strains.

Název v anglickém jazyce

Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains

Popis výsledku anglicky

The aim of this study was to compare the plasmid contents of methicillin-resistant Staphylococcus aureus (MRSA) strains classified into different clonal clusters (CCs). The isolates were collected from 15 Czech hospitals in 2000?2008. Plasmid DNA was detected in 65 (89%) strains and 33 of them harbored more than one plasmid type. Altogether 24 different types of plasmids were identified, ranging in size from 1.3 to 55 kb. Restriction endonuclease analysis, plasmid elimination, DNA hybridization, and sequencing were used for their further characterization. It has been found that the conjugative, erythromycin resistance and enterotoxin D encoding plasmids are harbored by strains from different CCs. On the other hand, chloramphenicol and tetracycline resistance plasmids, and most of the penicillinase and cryptic plasmids were only detected in certain CCs. Especially the pUSA300-like plasmids were found exclusively in the USA300 clone strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F09%2F0459" target="_blank" >GA310/09/0459: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Microbiology

ISSN

0302-8933

e-ISSN

—

Svazek periodika

194

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

607-614

Kód UT WoS článku

000305267200005

EID výsledku v databázi Scopus

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