MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00100669" target="_blank" >RIV/00216224:14310/18:00100669 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

Popis výsledku v původním jazyce

Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.

Název v anglickém jazyce

MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

Popis výsledku anglicky

Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/QJ1510216" target="_blank" >QJ1510216: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů