Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088403" target="_blank" >RIV/00216224:14310/16:00088403 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection

Popis výsledku v původním jazyce

Amino acids are very important compounds for living organisms. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Capillary electrophoresis offers conducting the derivatization reaction in pre-capillary, on capillary or post capillary arrangement. In the case of on-capillary derivatization, mixing of the reactants can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). This contribution deals with optimization of EMMA for derivatization of all standard AAs using naphthalene-2,3-dicarboxaldehyde and sodium cyanide and comparison with derivatization of AAs using TDLFP methodology.

Název v anglickém jazyce

Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection

Popis výsledku anglicky

Amino acids are very important compounds for living organisms. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Capillary electrophoresis offers conducting the derivatization reaction in pre-capillary, on capillary or post capillary arrangement. In the case of on-capillary derivatization, mixing of the reactants can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). This contribution deals with optimization of EMMA for derivatization of all standard AAs using naphthalene-2,3-dicarboxaldehyde and sodium cyanide and comparison with derivatization of AAs using TDLFP methodology.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Centrum pokročilých bioanalytických technologií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

13th International Interdisciplinary Meeting on Bioanalysis (CECE2016)

ISBN

9788090495944

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

100-103

Název nakladatele

Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic

Místo vydání

Brno, Czech Republic

Místo konání akce

Brno, Czech Republic

Datum konání akce

1. 1. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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