Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074190" target="_blank" >RIV/00216224:14310/14:00074190 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection

Popis výsledku v původním jazyce

Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution.

Název v anglickém jazyce

Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection

Popis výsledku anglicky

Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014

ISBN

9788090495920

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

154-157

Název nakladatele

Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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