Real-time PCR versus digital PCR in diagnostics of food-borne parasites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00091069" target="_blank" >RIV/00216224:14310/16:00091069 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Real-time PCR versus digital PCR in diagnostics of food-borne parasites

Popis výsledku v původním jazyce

In recent years, an increased number of reported cases of human infections by parasitic agents present in foodstuffs is recorded in Europe. Among the dominant pathogens are documented protozoa - Giardia intestinalis and Toxoplasma gondii. Cysts of G. intestinalis can survive long term in a cool and moist environment and contaminate e.g. fruit and vegetables growing in soil. Whereas T. gondii is an obligate intracellular parasite of warm-blooded animals, which forms tissue cysts in nervous and muscle tissue of the intermediate host; during the meat processing might be contaminated also tissue of uninfected hosts. Moreover, meat inspection of T. gondii at the slaughterhouses is not legislatively established. The detection possibilities of G. intestinalis and T. gondii were tested using two molecular methods - quantitative real-time PCR (qPCR) and digital PCR (dPCR).

Název v anglickém jazyce

Real-time PCR versus digital PCR in diagnostics of food-borne parasites

Popis výsledku anglicky

In recent years, an increased number of reported cases of human infections by parasitic agents present in foodstuffs is recorded in Europe. Among the dominant pathogens are documented protozoa - Giardia intestinalis and Toxoplasma gondii. Cysts of G. intestinalis can survive long term in a cool and moist environment and contaminate e.g. fruit and vegetables growing in soil. Whereas T. gondii is an obligate intracellular parasite of warm-blooded animals, which forms tissue cysts in nervous and muscle tissue of the intermediate host; during the meat processing might be contaminated also tissue of uninfected hosts. Moreover, meat inspection of T. gondii at the slaughterhouses is not legislatively established. The detection possibilities of G. intestinalis and T. gondii were tested using two molecular methods - quantitative real-time PCR (qPCR) and digital PCR (dPCR).

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů