The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00095481" target="_blank" >RIV/00216224:14310/17:00095481 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00706-017-2043-4" target="_blank" >https://link.springer.com/article/10.1007%2Fs00706-017-2043-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-017-2043-4" target="_blank" >10.1007/s00706-017-2043-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand

Popis výsledku v původním jazyce

The Eu(III) ternary complex with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (H(3)DO3A) and picolinate "antenna" ligand ([Eu(DO3A)(L)](-)) reacts with carbonate anions to form the more stable non-fluorescent [Eu(DO3A)(CO3)](2-) complex. This substitution reaction was employed for indirect determination of carbonate formed in course of urea hydrolysis catalyzed by the urease enzyme. This new analytical procedure was proposed for the determination of both analytes (urea, urease) and this sensitive luminescence-based biosensor can be used in both kinetic time-dependent and equilibrium end-point modes. This method, with analytical figures of merit (limit of detection 0.05 mM, dynamic range 0.05-5.00 mM), does not show any systematic error and therefore can be utilized for urea analysis in biological samples (e.g., urine). It was also demonstrated that some metal ions (e.g., Zn(II), Pb(II), Cd(II), Ag(I), etc.) inhibit the enzymatic hydrolysis of urea and this effect can be utilized for their determination; therefore, it is recommended to add EDTA as masking agent prior to enzymatic urea analysis.

Název v anglickém jazyce

The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand

Popis výsledku anglicky

The Eu(III) ternary complex with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (H(3)DO3A) and picolinate "antenna" ligand ([Eu(DO3A)(L)](-)) reacts with carbonate anions to form the more stable non-fluorescent [Eu(DO3A)(CO3)](2-) complex. This substitution reaction was employed for indirect determination of carbonate formed in course of urea hydrolysis catalyzed by the urease enzyme. This new analytical procedure was proposed for the determination of both analytes (urea, urease) and this sensitive luminescence-based biosensor can be used in both kinetic time-dependent and equilibrium end-point modes. This method, with analytical figures of merit (limit of detection 0.05 mM, dynamic range 0.05-5.00 mM), does not show any systematic error and therefore can be utilized for urea analysis in biological samples (e.g., urine). It was also demonstrated that some metal ions (e.g., Zn(II), Pb(II), Cd(II), Ag(I), etc.) inhibit the enzymatic hydrolysis of urea and this effect can be utilized for their determination; therefore, it is recommended to add EDTA as masking agent prior to enzymatic urea analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

148

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

1945-1952

Kód UT WoS článku

000413626000008

EID výsledku v databázi Scopus

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