Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00111923" target="_blank" >RIV/00216224:14310/17:00111923 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/17:39911343

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1359511316310613" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1359511316310613</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.procbio.2017.04.016" target="_blank" >10.1016/j.procbio.2017.04.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties

Popis výsledku v původním jazyce

In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100 Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078 IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG.

Název v anglickém jazyce

Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties

Popis výsledku anglicky

In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100 Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078 IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Process Biochemistry

ISSN

1359-5113

e-ISSN

1873-3298

Svazek periodika

58

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

313-319

Kód UT WoS článku

000404710700042

EID výsledku v databázi Scopus

2-s2.0-85018570879