Computational Analysis of Protein Tunnels and Channels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00102092" target="_blank" >RIV/00216224:14310/18:00102092 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-7366-8_3" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7366-8_3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7366-8_3" target="_blank" >10.1007/978-1-4939-7366-8_3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Computational Analysis of Protein Tunnels and Channels

Popis výsledku v původním jazyce

Protein tunnels connecting the functional buried cavities with bulk solvent and protein channels, enabling the transport through biological membranes, represent the structural features that govern the exchange rates of ligands, ions, and water solvent. Tunnels and channels are present in a vast number of known proteins and provide control over their function. Modification of these structural features by protein engineering frequently provides proteins with improved properties. Here we present a detailed computational protocol employing the CAVER software that is applicable for: (1) the analysis of tunnels and channels in protein structures, and (2) the selection of hot-spot residues in tunnels or channels that can be mutagenized for improved activity, specificity, enantioselectivity, or stability.

Název v anglickém jazyce

Computational Analysis of Protein Tunnels and Channels

Popis výsledku anglicky

Protein tunnels connecting the functional buried cavities with bulk solvent and protein channels, enabling the transport through biological membranes, represent the structural features that govern the exchange rates of ligands, ions, and water solvent. Tunnels and channels are present in a vast number of known proteins and provide control over their function. Modification of these structural features by protein engineering frequently provides proteins with improved properties. Here we present a detailed computational protocol employing the CAVER software that is applicable for: (1) the analysis of tunnels and channels in protein structures, and (2) the selection of hot-spot residues in tunnels or channels that can be mutagenized for improved activity, specificity, enantioselectivity, or stability.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

20400 - Chemical engineering

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Protein Engineering. Methods in Molecular Biology

ISBN

9781493973644

Počet stran výsledku

18

Strana od-do

25-42

Počet stran knihy

350

Název nakladatele

Humana Press

Místo vydání

New York, NY

Kód UT WoS kapitoly

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