Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107656" target="_blank" >RIV/00216224:14310/19:00107656 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity

Popis výsledku v původním jazyce

Pharmaceuticals and pharmaceutically active substances nowadays represent an important class of emerging environmental pollutants. Growth and developmental changes in plants induced by pharmaceuticals reflect changes of processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity. Non-steroidal anti-inflammatory drug diclofenac (DCF) belongs to currently monitored compounds in the environment because of its frequent occurrence and high potential toxicity. This study suggests that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, suspension density and mitotic index. The growth and viability changes after 120 h of treatment were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide, and by down-regulation of reactive nitrogen species and total thiols in correlation to DCF treatment. DCF-induced oxidative stress triggered apoptotic alterations in BY-2 cells leading to programmed cell death – irregular or stretched nuclei were observed in up to 50% of cells. The response of the tobacco BY-2 cell suspension to DCF treatment is prompt and sensitive and selected parameters are suitable for toxicity evaluation.

Název v anglickém jazyce

Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity

Popis výsledku anglicky

Pharmaceuticals and pharmaceutically active substances nowadays represent an important class of emerging environmental pollutants. Growth and developmental changes in plants induced by pharmaceuticals reflect changes of processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity. Non-steroidal anti-inflammatory drug diclofenac (DCF) belongs to currently monitored compounds in the environment because of its frequent occurrence and high potential toxicity. This study suggests that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, suspension density and mitotic index. The growth and viability changes after 120 h of treatment were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide, and by down-regulation of reactive nitrogen species and total thiols in correlation to DCF treatment. DCF-induced oxidative stress triggered apoptotic alterations in BY-2 cells leading to programmed cell death – irregular or stretched nuclei were observed in up to 50% of cells. The response of the tobacco BY-2 cell suspension to DCF treatment is prompt and sensitive and selected parameters are suitable for toxicity evaluation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GF17-33746L" target="_blank" >GF17-33746L: Léčiva v životním prostředí a interakce s rostlinami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů