Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107657" target="_blank" >RIV/00216224:14310/19:00107657 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact

Popis výsledku v původním jazyce

Studies focusing on environmental contamination have shown that besides “classic” persistent pollutants, pseudo-persistent emerging contaminants are also present. As highly specific biologically active substances, pharmaceuticals and pharmaceutically active substances entering the environment can influence non-target organisms including plants. One of the commonly detected and monitored pharmaceuticals in water is a non-steroidal anti-inflammatory drug diclofenac (DCF). Plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity of wide range of inorganic and organic pollutants at the cellular and subcellular levels. The question is, if suspension BY-2 can be used even for drug toxicity evaluation. Results of this study suggest that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, mitotic index, suspension density and dry weight. DCF-induced oxidative stress documented by increased production of total reactive oxygen species (ROS) triggered apoptotic alterations in BY-2 cells leading to programmed cell death – occurrence of acidic compartments and autophagic vacuoles in BY-2 cells and apoptotic chromatin condensation and DNA fragmentation in their nuclei. Due to its prompt and sensitive response tobacco BY-2 cell suspension is suitable for evaluation of phytotoxicity of drugs.

Název v anglickém jazyce

Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact

Popis výsledku anglicky

Studies focusing on environmental contamination have shown that besides “classic” persistent pollutants, pseudo-persistent emerging contaminants are also present. As highly specific biologically active substances, pharmaceuticals and pharmaceutically active substances entering the environment can influence non-target organisms including plants. One of the commonly detected and monitored pharmaceuticals in water is a non-steroidal anti-inflammatory drug diclofenac (DCF). Plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity of wide range of inorganic and organic pollutants at the cellular and subcellular levels. The question is, if suspension BY-2 can be used even for drug toxicity evaluation. Results of this study suggest that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, mitotic index, suspension density and dry weight. DCF-induced oxidative stress documented by increased production of total reactive oxygen species (ROS) triggered apoptotic alterations in BY-2 cells leading to programmed cell death – occurrence of acidic compartments and autophagic vacuoles in BY-2 cells and apoptotic chromatin condensation and DNA fragmentation in their nuclei. Due to its prompt and sensitive response tobacco BY-2 cell suspension is suitable for evaluation of phytotoxicity of drugs.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GF17-33746L" target="_blank" >GF17-33746L: Léčiva v životním prostředí a interakce s rostlinami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů