Interaction of selenite with metallothionein studied by Brdicka reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00110355" target="_blank" >RIV/00216224:14310/19:00110355 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00706-019-02397-4" target="_blank" >https://link.springer.com/article/10.1007/s00706-019-02397-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-02397-4" target="_blank" >10.1007/s00706-019-02397-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of selenite with metallothionein studied by Brdicka reaction

Popis výsledku v původním jazyce

Metallothionein II (MT) is a cytosolic, ubiquitous, low-molecular-weight protein present in various tissues of mammals and non-mammals. MTs have a high content of thiol groups (-SH) which can bind mineral micronutrients and xenobiotic heavy metals. In this study, we investigated the electrode processes of MT at a mercury electrode in the presence of sodium selenite by means of the Brdika reaction in the differential pulse voltammetric mode. In Brdika solution containing ammonium buffer and [Co(NH3)(6)]Cl-3, the substance that provides catalytic reactions with proteins, interactions between MT and sodium selenite were analyzed via the hydrogen evolution catalytic signals. The reaction of -SH groups of MT with [Co(H2O)(6)](2+) complex ions on the mercury surface results into the catalytic peak Cat2. It was found that selenite ions are active in the Brdika reaction and yield also the catalytic peak of hydrogen evolution Cat2. With the increasing concentration of sodium selenite, cobalt (Co) in MT is replaced by selenium (Se). When selenite concentration increases above the MT binding capacity, i.e., MT cannot bind more Se, only selenite ions can be responsible for Cat2 signals. From our results, we conclude that Brdika reaction is a suitable method to study MT binding properties and to evaluate the influence of sodium selenite. To elucidate the experimental results a new interpretation leading to complete description of the mechanism is presented.

Název v anglickém jazyce

Interaction of selenite with metallothionein studied by Brdicka reaction

Popis výsledku anglicky

Metallothionein II (MT) is a cytosolic, ubiquitous, low-molecular-weight protein present in various tissues of mammals and non-mammals. MTs have a high content of thiol groups (-SH) which can bind mineral micronutrients and xenobiotic heavy metals. In this study, we investigated the electrode processes of MT at a mercury electrode in the presence of sodium selenite by means of the Brdika reaction in the differential pulse voltammetric mode. In Brdika solution containing ammonium buffer and [Co(NH3)(6)]Cl-3, the substance that provides catalytic reactions with proteins, interactions between MT and sodium selenite were analyzed via the hydrogen evolution catalytic signals. The reaction of -SH groups of MT with [Co(H2O)(6)](2+) complex ions on the mercury surface results into the catalytic peak Cat2. It was found that selenite ions are active in the Brdika reaction and yield also the catalytic peak of hydrogen evolution Cat2. With the increasing concentration of sodium selenite, cobalt (Co) in MT is replaced by selenium (Se). When selenite concentration increases above the MT binding capacity, i.e., MT cannot bind more Se, only selenite ions can be responsible for Cat2 signals. From our results, we conclude that Brdika reaction is a suitable method to study MT binding properties and to evaluate the influence of sodium selenite. To elucidate the experimental results a new interpretation leading to complete description of the mechanism is presented.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/LM2015041" target="_blank" >LM2015041: CEITEC Nano</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie-Chemical Monthly

ISSN

0026-9247

e-ISSN

1434-4475

Svazek periodika

150

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

7

Strana od-do

469-475

Kód UT WoS článku

000461394400014

EID výsledku v databázi Scopus

2-s2.0-85062717120