Interaction of selenite with metallothionein studied by Brdika reaction
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134877" target="_blank" >RIV/00216305:26620/19:PU134877 - isvavai.cz</a>
Výsledek na webu
<a href="https://link.springer.com/article/10.1007/s00706-019-02397-4" target="_blank" >https://link.springer.com/article/10.1007/s00706-019-02397-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-019-02397-4" target="_blank" >10.1007/s00706-019-02397-4</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Interaction of selenite with metallothionein studied by Brdika reaction
Popis výsledku v původním jazyce
Metallothionein II (MT) is a cytosolic, ubiquitous, low-molecular-weight protein present in various tissues of mammals and non-mammals. MTs have a high content of thiol groups (-SH) which can bind mineral micronutrients and xenobiotic heavy metals. In this study, we investigated the electrode processes of MT at a mercury electrode in the presence of sodium selenite by means of the Brdika reaction in the differential pulse voltammetric mode. In Brdika solution containing ammonium buffer and [Co(NH3)(6)]Cl-3, the substance that provides catalytic reactions with proteins, interactions between MT and sodium selenite were analyzed via the hydrogen evolution catalytic signals. The reaction of -SH groups of MT with [Co(H2O)(6)](2+) complex ions on the mercury surface results into the catalytic peak Cat2. It was found that selenite ions are active in the Brdika reaction and yield also the catalytic peak of hydrogen evolution Cat2. With the increasing concentration of sodium selenite, cobalt (Co) in MT is replaced by selenium (Se). When selenite concentration increases above the MT binding capacity, i.e., MT cannot bind more Se, only selenite ions can be responsible for Cat2 signals. From our results, we conclude that Brdika reaction is a suitable method to study MT binding properties and to evaluate the influence of sodium selenite. To elucidate the experimental results a new interpretation leading to complete description of the mechanism is presented. [GRAPHICS] .
Název v anglickém jazyce
Interaction of selenite with metallothionein studied by Brdika reaction
Popis výsledku anglicky
Metallothionein II (MT) is a cytosolic, ubiquitous, low-molecular-weight protein present in various tissues of mammals and non-mammals. MTs have a high content of thiol groups (-SH) which can bind mineral micronutrients and xenobiotic heavy metals. In this study, we investigated the electrode processes of MT at a mercury electrode in the presence of sodium selenite by means of the Brdika reaction in the differential pulse voltammetric mode. In Brdika solution containing ammonium buffer and [Co(NH3)(6)]Cl-3, the substance that provides catalytic reactions with proteins, interactions between MT and sodium selenite were analyzed via the hydrogen evolution catalytic signals. The reaction of -SH groups of MT with [Co(H2O)(6)](2+) complex ions on the mercury surface results into the catalytic peak Cat2. It was found that selenite ions are active in the Brdika reaction and yield also the catalytic peak of hydrogen evolution Cat2. With the increasing concentration of sodium selenite, cobalt (Co) in MT is replaced by selenium (Se). When selenite concentration increases above the MT binding capacity, i.e., MT cannot bind more Se, only selenite ions can be responsible for Cat2 signals. From our results, we conclude that Brdika reaction is a suitable method to study MT binding properties and to evaluate the influence of sodium selenite. To elucidate the experimental results a new interpretation leading to complete description of the mechanism is presented. [GRAPHICS] .
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Monatshefte für Chemie
ISSN
0026-9247
e-ISSN
1434-4475
Svazek periodika
150
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
AT - Rakouská republika
Počet stran výsledku
7
Strana od-do
469-475
Kód UT WoS článku
000461394400014
EID výsledku v databázi Scopus
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