Determination of Riboflavin by Nanocomposite Modified Carbon Paste Electrode in Biological Fluids Using Fast Fourier Transform Square Wave Voltammetry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00117223" target="_blank" >RIV/00216224:14310/20:00117223 - isvavai.cz</a>

Výsledek na webu

<a href="https://dx.doi.org/10.5829/ije.2020.33.09c.01" target="_blank" >https://dx.doi.org/10.5829/ije.2020.33.09c.01</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5829/ije.2020.33.09c.01" target="_blank" >10.5829/ije.2020.33.09c.01</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Riboflavin by Nanocomposite Modified Carbon Paste Electrode in Biological Fluids Using Fast Fourier Transform Square Wave Voltammetry

Popis výsledku v původním jazyce

Herein, fast Fourier transformation square-wave voltammetry (FFT-SWV) as a novel electrochemical determination technique was used to investigate the electrochemical behavior and determination of Riboflavin at the surface of a nanocomposite modified carbon paste electrode. The carbon paste electrode was modified by nanocomposite containing Samarium oxide (Sm2O3)/reduced graphene oxide (RGO) (2:1) to improve detection sensitivity of Riboflavin under optimal experimental conditions. Furthermore, the signal-to-noise ratio was significantly increased by application of discrete fast Fourier transformation analysis, background subtraction and two-dimensional integration of the electrode response over the selected potential range and the time window. Obtained cyclic voltammograms demonstrated a diffusion-controlled reversible electron transfer reaction for Riboflavin in phosphate buffer solution (pH=7.2). The peak potential values were pH-dependent, involving the same numbers of protons and electrons. To obtain the maximum sensitivity, some effective parameters such as scan rate (10 mV/s), accumulation time (0.2 s) and potential (+400 mV), frequency (1420 Hz) and amplitude (20 mV) were optimized. As a result, determination of Riboflavin using FFT-SWV showed a linear range of response from 10 to 400 nM (R-2=0.9993), with limit of detection of 0.86 nM. An acceptable recovery percent was also obtained for Riboflavin in human plasma samples as criteria of measurement applicability of the proposed modified electrode.

Název v anglickém jazyce

Determination of Riboflavin by Nanocomposite Modified Carbon Paste Electrode in Biological Fluids Using Fast Fourier Transform Square Wave Voltammetry

Popis výsledku anglicky

Herein, fast Fourier transformation square-wave voltammetry (FFT-SWV) as a novel electrochemical determination technique was used to investigate the electrochemical behavior and determination of Riboflavin at the surface of a nanocomposite modified carbon paste electrode. The carbon paste electrode was modified by nanocomposite containing Samarium oxide (Sm2O3)/reduced graphene oxide (RGO) (2:1) to improve detection sensitivity of Riboflavin under optimal experimental conditions. Furthermore, the signal-to-noise ratio was significantly increased by application of discrete fast Fourier transformation analysis, background subtraction and two-dimensional integration of the electrode response over the selected potential range and the time window. Obtained cyclic voltammograms demonstrated a diffusion-controlled reversible electron transfer reaction for Riboflavin in phosphate buffer solution (pH=7.2). The peak potential values were pH-dependent, involving the same numbers of protons and electrons. To obtain the maximum sensitivity, some effective parameters such as scan rate (10 mV/s), accumulation time (0.2 s) and potential (+400 mV), frequency (1420 Hz) and amplitude (20 mV) were optimized. As a result, determination of Riboflavin using FFT-SWV showed a linear range of response from 10 to 400 nM (R-2=0.9993), with limit of detection of 0.86 nM. An acceptable recovery percent was also obtained for Riboflavin in human plasma samples as criteria of measurement applicability of the proposed modified electrode.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Engineering

ISSN

2423-7167

e-ISSN

1735-9244

Svazek periodika

33

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

IR - Íránská islámská republika

Počet stran výsledku

7

Strana od-do

1696-1702

Kód UT WoS článku

000577917800001

EID výsledku v databázi Scopus

2-s2.0-85091658949