Maintenance of Aquatic Hyphomycete Cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00117435" target="_blank" >RIV/00216224:14310/20:00117435 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-3-030-30515-4_24" target="_blank" >https://doi.org/10.1007/978-3-030-30515-4_24</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-030-30515-4_24" target="_blank" >10.1007/978-3-030-30515-4_24</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Maintenance of Aquatic Hyphomycete Cultures

Popis výsledku v původním jazyce

Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen.

Název v anglickém jazyce

Maintenance of Aquatic Hyphomycete Cultures

Popis výsledku anglicky

Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10612 - Mycology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Methods to Study Litter Decomposition

ISBN

9783030305147

Počet stran výsledku

12

Strana od-do

211-222

Počet stran knihy

604

Název nakladatele

Springer

Místo vydání

Cham

Kód UT WoS kapitoly

—