Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00119125" target="_blank" >RIV/00216224:14310/21:00119125 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/21:00544056 RIV/00209805:_____/21:00078964 RIV/00216208:11310/21:10434284

Výsledek na webu

<a href="https://doi.org/10.3354/dao03601" target="_blank" >https://doi.org/10.3354/dao03601</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3354/dao03601" target="_blank" >10.3354/dao03601</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'

Popis výsledku v původním jazyce

The swim bladder inflates early after fish hatching via its interconnection with the digestive tract (ductus pneumaticus). This interconnection may serve as a portal to foreign particles, including bacteria, causing deficiencies in primary swim bladder inflation. We histologically examined 134 African annual killifish (genus Nothobranchius) with secondary loss of swim bladder function ('belly sliders'). We demonstrate that these fish lost the ability of air regulation in their swim bladders likely due to Mycobacterium spp. infection at an individual-specific age. Nearly all examined belly sliders had thickened swim bladder walls, and their swim bladder was filled with material containing mycobacteria, cell debris, young monocytic cells and phagocyting macrophages. Mycobacterial infection was restricted to the swim bladder in juveniles, where mycobacteria likely enter the host through the ductus pneumaticus. Infection in adults was systemic and mycobacteria were present in all examined organs. Presence of mycobacteria in the epithelial lining and submucosal layers of the digestive tract of adults suggests that it may also serve as the entrance site of infection. We suspect 2 sources of Mycobacterium contamination: dietary (with bloodworms) and/or contaminated hatching substrate. These sources of contamination may be eliminated by use of laboratory dry feed and egg disinfection prior to hatching.

Název v anglickém jazyce

Swim bladder as a primary site of mycobacterial infection in Nothobranchius 'belly sliders'

Popis výsledku anglicky

The swim bladder inflates early after fish hatching via its interconnection with the digestive tract (ductus pneumaticus). This interconnection may serve as a portal to foreign particles, including bacteria, causing deficiencies in primary swim bladder inflation. We histologically examined 134 African annual killifish (genus Nothobranchius) with secondary loss of swim bladder function ('belly sliders'). We demonstrate that these fish lost the ability of air regulation in their swim bladders likely due to Mycobacterium spp. infection at an individual-specific age. Nearly all examined belly sliders had thickened swim bladder walls, and their swim bladder was filled with material containing mycobacteria, cell debris, young monocytic cells and phagocyting macrophages. Mycobacterial infection was restricted to the swim bladder in juveniles, where mycobacteria likely enter the host through the ductus pneumaticus. Infection in adults was systemic and mycobacteria were present in all examined organs. Presence of mycobacteria in the epithelial lining and submucosal layers of the digestive tract of adults suggests that it may also serve as the entrance site of infection. We suspect 2 sources of Mycobacterium contamination: dietary (with bloodworms) and/or contaminated hatching substrate. These sources of contamination may be eliminated by use of laboratory dry feed and egg disinfection prior to hatching.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA19-20873S" target="_blank" >GA19-20873S: Klíčové mutace v nádorovém genomu Nothobranchius furzeri: od nádorové biologie k návrhu experimentálního modelu sporadické kancerogeneze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diseases of aquatic organisms

ISSN

0177-5103

e-ISSN

1616-1580

Svazek periodika

145

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

111-117

Kód UT WoS článku

000691780800010

EID výsledku v databázi Scopus

2-s2.0-85104155322