Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F23%3A00570608" target="_blank" >RIV/68081766:_____/23:00570608 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00130644

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10641-023-01405-1" target="_blank" >https://link.springer.com/article/10.1007/s10641-023-01405-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10641-023-01405-1" target="_blank" >10.1007/s10641-023-01405-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

Popis výsledku v původním jazyce

Providing optimal conditions for early-life gas bladder inflation of captive fish is one of the biggest challenges in fish culture. It also applies to laboratory fishes. Turquoise killifish (Nothobranchius furzeri Jubb, 1971) is a popular research model in biogerontology due to its short lifespan. Annual killifish in laboratory culture frequently suffer from an inability to inflate their gas bladder which may stem from suboptimal environmental conditions in captivity. Here, we investigate (1) the effect of dissolved oxygen (DO) saturation and (2) access to the water surface on gas bladder inflation and hatching success of turquoise killifish. We further histologically examine the gas bladder development from its primordial form to full inflation. In accordance with physoclistous nature of turquoise killifish, access to the water surface is not necessary for gas bladder inflation. We found that hatching success was highest in the treatment with constant or decreasing DO saturation. In contrast, the highest proportion of larvae with inflated gas bladders was found in the treatment with DO oversaturated water (130%) which was induced by the addition of an oxygen tablet. Larvae inflated their gas bladders within 2 to 48 h post-hatching. These findings represent a major step toward a solution to a persistent problem in laboratory culture of this increasingly important model organism.

Název v anglickém jazyce

Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

Popis výsledku anglicky

Providing optimal conditions for early-life gas bladder inflation of captive fish is one of the biggest challenges in fish culture. It also applies to laboratory fishes. Turquoise killifish (Nothobranchius furzeri Jubb, 1971) is a popular research model in biogerontology due to its short lifespan. Annual killifish in laboratory culture frequently suffer from an inability to inflate their gas bladder which may stem from suboptimal environmental conditions in captivity. Here, we investigate (1) the effect of dissolved oxygen (DO) saturation and (2) access to the water surface on gas bladder inflation and hatching success of turquoise killifish. We further histologically examine the gas bladder development from its primordial form to full inflation. In accordance with physoclistous nature of turquoise killifish, access to the water surface is not necessary for gas bladder inflation. We found that hatching success was highest in the treatment with constant or decreasing DO saturation. In contrast, the highest proportion of larvae with inflated gas bladders was found in the treatment with DO oversaturated water (130%) which was induced by the addition of an oxygen tablet. Larvae inflated their gas bladders within 2 to 48 h post-hatching. These findings represent a major step toward a solution to a persistent problem in laboratory culture of this increasingly important model organism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA18-26284S" target="_blank" >GA18-26284S: Embryo a jeho prostředí - anuální ryby jako unikátní model pro studium embryonální ekologie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Biology of Fishes

ISSN

0378-1909

e-ISSN

1573-5133

Svazek periodika

106

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

673-683

Kód UT WoS článku

000949110800001

EID výsledku v databázi Scopus

2-s2.0-85149970550