Effect of a non-thermal atmospheric pressure plasma jet on four different yeasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00120795" target="_blank" >RIV/00216224:14310/21:00120795 - isvavai.cz</a>

Výsledek na webu

<a href="https://iopscience.iop.org/article/10.1088/1361-6463/abb624" target="_blank" >https://iopscience.iop.org/article/10.1088/1361-6463/abb624</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6463/abb624" target="_blank" >10.1088/1361-6463/abb624</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of a non-thermal atmospheric pressure plasma jet on four different yeasts

Popis výsledku v původním jazyce

Non-thermal atmospheric pressure plasmas are known to be an effective method for the inactivation of microorganisms. However, yeasts have proved to be more resistant to provided treatments. We investigated the influence of an Argon plasma jet on the inactivation of four different yeasts; Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida parapsilosis and Magnusiomyces magnusii. Results from direct plasma treatment on the yeasts in distilled water, as the most effective technique we found, are presented here. The surviving yeasts after 5, 7, and 10 min plasma exposures were evaluated visually and quantitatively. Quantitative results showed strong reductions in the survival rates after the plasma treatment. The longest plasma exposure time yielded the least survival. Moreover, how long the inactivations lasted was evaluated by assessing the treated samples after 1 h and again after 2 h. The survival rates were further decreased within these storage times. In the 10 min treated-2 h stored samples, the rates dropped to 5% or even below, depending on the yeast species. High concentrations of reactive oxygen and nitrogen species in the plasma-air-water interacting system were detected by optical emission spectroscopy of the plasma jet and also by measuring concentrations of H2O2, NO2- and NO3- in plasma activated water (as the surrounding liquid for the yeasts). The conductivity and pH of the treated water was measured as well. The level of the conductivity increased along with the decrease in pH. Synergistic effects of these chemical reactive species in acidified water resulted in the yeast inactivation.

Název v anglickém jazyce

Effect of a non-thermal atmospheric pressure plasma jet on four different yeasts

Popis výsledku anglicky

Non-thermal atmospheric pressure plasmas are known to be an effective method for the inactivation of microorganisms. However, yeasts have proved to be more resistant to provided treatments. We investigated the influence of an Argon plasma jet on the inactivation of four different yeasts; Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida parapsilosis and Magnusiomyces magnusii. Results from direct plasma treatment on the yeasts in distilled water, as the most effective technique we found, are presented here. The surviving yeasts after 5, 7, and 10 min plasma exposures were evaluated visually and quantitatively. Quantitative results showed strong reductions in the survival rates after the plasma treatment. The longest plasma exposure time yielded the least survival. Moreover, how long the inactivations lasted was evaluated by assessing the treated samples after 1 h and again after 2 h. The survival rates were further decreased within these storage times. In the 10 min treated-2 h stored samples, the rates dropped to 5% or even below, depending on the yeast species. High concentrations of reactive oxygen and nitrogen species in the plasma-air-water interacting system were detected by optical emission spectroscopy of the plasma jet and also by measuring concentrations of H2O2, NO2- and NO3- in plasma activated water (as the surrounding liquid for the yeasts). The conductivity and pH of the treated water was measured as well. The level of the conductivity increased along with the decrease in pH. Synergistic effects of these chemical reactive species in acidified water resulted in the yeast inactivation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of physics D: Applied physics

ISSN

0022-3727

e-ISSN

1361-6463

Svazek periodika

54

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000581600900001

EID výsledku v databázi Scopus

2-s2.0-85094956493