Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00122724" target="_blank" >RIV/00216224:14310/21:00122724 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jasrep.2021.103216" target="_blank" >https://doi.org/10.1016/j.jasrep.2021.103216</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jasrep.2021.103216" target="_blank" >10.1016/j.jasrep.2021.103216</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

Popis výsledku v původním jazyce

Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.

Název v anglickém jazyce

Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

Popis výsledku anglicky

Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Archaeological Science: Reports

ISSN

2352-409X

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

000714601300002

EID výsledku v databázi Scopus

2-s2.0-85118339329