Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00131176" target="_blank" >RIV/00216224:14310/23:00131176 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

Popis výsledku v původním jazyce

Inhalation injury (INHI) is defined as an acute airway injury caused by inhalation of hot steam and/or products of combustion. Mortality in patients with INHI is often associated with the development of secondary complications such as extensive infections, pneumonia, direct mucosal damage, mucosal sloughing, and obstructions. Currently, it is acknowledged that the major problem lies in the lack of uniformity in diagnosis, grading, and treatment. The investigation of specific biomarkers for INHI has a great potential to address this issue. Such biomarkers could serve not only to unify diagnosis and treatment, but also to predict the development of secondary complications in patients with INHI. Due to the rarity of the injury, this complex project was designed as a prospective study with the aim of monitoring individual patient parameters during hospitalization. Samples of several matrices (buccal and oropharyngeal swabs, bronchoalveolar lavage - BAL, urine, and blood) for subsequent analyses are collected at 6 time points. In my thesis, I focus on the molecular biological aspects of INHI. Therefore, the main aims are: i) to determine the microbiome and antibiotic resistance genes in samples from upper and lower airways, blood, and/or urine of patients with INHI and with/without infectious complications during their hospitalization; and ii) to select miRNA correlating with INHI using RNAseq analysis in BAL and urine samples, and iii) to characterize toxic compound in BAL and urine.

Název v anglickém jazyce

Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

Popis výsledku anglicky

Inhalation injury (INHI) is defined as an acute airway injury caused by inhalation of hot steam and/or products of combustion. Mortality in patients with INHI is often associated with the development of secondary complications such as extensive infections, pneumonia, direct mucosal damage, mucosal sloughing, and obstructions. Currently, it is acknowledged that the major problem lies in the lack of uniformity in diagnosis, grading, and treatment. The investigation of specific biomarkers for INHI has a great potential to address this issue. Such biomarkers could serve not only to unify diagnosis and treatment, but also to predict the development of secondary complications in patients with INHI. Due to the rarity of the injury, this complex project was designed as a prospective study with the aim of monitoring individual patient parameters during hospitalization. Samples of several matrices (buccal and oropharyngeal swabs, bronchoalveolar lavage - BAL, urine, and blood) for subsequent analyses are collected at 6 time points. In my thesis, I focus on the molecular biological aspects of INHI. Therefore, the main aims are: i) to determine the microbiome and antibiotic resistance genes in samples from upper and lower airways, blood, and/or urine of patients with INHI and with/without infectious complications during their hospitalization; and ii) to select miRNA correlating with INHI using RNAseq analysis in BAL and urine samples, and iii) to characterize toxic compound in BAL and urine.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů