Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00133529" target="_blank" >RIV/00216224:14310/23:00133529 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

Popis výsledku v původním jazyce

Background and Aims Inhalation injury (INHI) is defined as acute airway injury caused by inhalation of hot steam and/or products of combustion. During the hospitalization of these patients, commensal bacterial populations colonizing the lungs, such as Prevotella spp. and Veillonella spp., are displaced by bacteria with pathogenic potential, such as Pseudomonas aeruginosa and Klebsiella pneumonia. In our pilot study, we aimed to determine the dynamic changes of the bacteriome in 10 patients with INHI during their hospitalization. Methods We characterized the bacteriome from oral and oropharyngeal swabs, bronchoalveolar lavage (BAL), catheter urine, and blood using 16S rDNA sequencing at 6 time-points of hospitalization (Days 1 - 28). Results Oral and oropharyngeal swabs revealed high abundance of bacterial DNA. The bacterial composition was quite stable among the studied time-points, and both their alpha- and beta-diversities were similar in similar matrices. The BAL bacteriome composition was related to the oral bacteriome of respective patients; high abundances of Klebsiella sp., Enterobacter sp., Haemophillus sp., Escherichia sp., Staphylococcus sp., Pseudomonas sp., and Neisseria sp. were found in BAL. The results showed that the urine and blood samples were almost sterile at the beginning of the hospitalization; however, in some cases, low amounts of Klebsiella sp. and high abundance of Proteus sp. or Escherichia sp. were found in the blood and urine samples, respectively. Conclusion To conclude, the oral bacteriome seems to be a source of bacterial lung infection in patients with INHI during their hospitalization. Therefore, the oral microbiota could serve as potential a screening marker for the lung microbiome.

Název v anglickém jazyce

Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

Popis výsledku anglicky

Background and Aims Inhalation injury (INHI) is defined as acute airway injury caused by inhalation of hot steam and/or products of combustion. During the hospitalization of these patients, commensal bacterial populations colonizing the lungs, such as Prevotella spp. and Veillonella spp., are displaced by bacteria with pathogenic potential, such as Pseudomonas aeruginosa and Klebsiella pneumonia. In our pilot study, we aimed to determine the dynamic changes of the bacteriome in 10 patients with INHI during their hospitalization. Methods We characterized the bacteriome from oral and oropharyngeal swabs, bronchoalveolar lavage (BAL), catheter urine, and blood using 16S rDNA sequencing at 6 time-points of hospitalization (Days 1 - 28). Results Oral and oropharyngeal swabs revealed high abundance of bacterial DNA. The bacterial composition was quite stable among the studied time-points, and both their alpha- and beta-diversities were similar in similar matrices. The BAL bacteriome composition was related to the oral bacteriome of respective patients; high abundances of Klebsiella sp., Enterobacter sp., Haemophillus sp., Escherichia sp., Staphylococcus sp., Pseudomonas sp., and Neisseria sp. were found in BAL. The results showed that the urine and blood samples were almost sterile at the beginning of the hospitalization; however, in some cases, low amounts of Klebsiella sp. and high abundance of Proteus sp. or Escherichia sp. were found in the blood and urine samples, respectively. Conclusion To conclude, the oral bacteriome seems to be a source of bacterial lung infection in patients with INHI during their hospitalization. Therefore, the oral microbiota could serve as potential a screening marker for the lung microbiome.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů