High-Throughput Microbore LC-MS Lipidomics to Investigate APOE Phenotypes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00136659" target="_blank" >RIV/00216224:14310/24:00136659 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.3c02652" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.3c02652</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.3c02652" target="_blank" >10.1021/acs.analchem.3c02652</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-Throughput Microbore LC-MS Lipidomics to Investigate APOE Phenotypes

Popis výsledku v původním jazyce

Microflow liquid chromatography interfaced with mass spectrometry (mu LC-MS/MS) is increasingly applied for high-throughput profiling of biological samples and has been proven to have an acceptable trade-off between sensitivity and reproducibility. However, lipidomics applications are scarce. We optimized a mu LC-MS/MS system utilizing a 1 mm inner diameter x 100 mm column coupled to a triple quadrupole mass spectrometer to establish a sensitive, high-throughput, and robust single-shot lipidomics workflow. Compared to conventional lipidomics methods, we achieve a similar to 4-fold increase in response, facilitating quantification of 351 lipid species from a single iPSC-derived cerebral organoid during a 15 min LC-MS analysis. Consecutively, we injected 303 samples over similar to 75 h to prove the robustness and reproducibility of the microflow separation. As a proof of concept, mu LC-MS/MS analysis of Alzheimer's disease patient-derived iPSC cerebral organoid reveals differential lipid metabolism depending on APOE phenotype (E3/3 vs E4/4). Microflow separation proves to be an environmentally friendly and cost-effective method as it reduces the consumption of harmful solvents. Also, the data demonstrate robust, in-depth, high-throughput performance to enable routine clinical or biomedical applications.

Název v anglickém jazyce

High-Throughput Microbore LC-MS Lipidomics to Investigate APOE Phenotypes

Popis výsledku anglicky

Microflow liquid chromatography interfaced with mass spectrometry (mu LC-MS/MS) is increasingly applied for high-throughput profiling of biological samples and has been proven to have an acceptable trade-off between sensitivity and reproducibility. However, lipidomics applications are scarce. We optimized a mu LC-MS/MS system utilizing a 1 mm inner diameter x 100 mm column coupled to a triple quadrupole mass spectrometer to establish a sensitive, high-throughput, and robust single-shot lipidomics workflow. Compared to conventional lipidomics methods, we achieve a similar to 4-fold increase in response, facilitating quantification of 351 lipid species from a single iPSC-derived cerebral organoid during a 15 min LC-MS analysis. Consecutively, we injected 303 samples over similar to 75 h to prove the robustness and reproducibility of the microflow separation. As a proof of concept, mu LC-MS/MS analysis of Alzheimer's disease patient-derived iPSC cerebral organoid reveals differential lipid metabolism depending on APOE phenotype (E3/3 vs E4/4). Microflow separation proves to be an environmentally friendly and cost-effective method as it reduces the consumption of harmful solvents. Also, the data demonstrate robust, in-depth, high-throughput performance to enable routine clinical or biomedical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

96

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

59-66

Kód UT WoS článku

001139472200001

EID výsledku v databázi Scopus

2-s2.0-85180563035