Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Popis výsledku v původním jazyce

Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvaticus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p&gt;0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex. The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.

Název v anglickém jazyce

Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Popis výsledku anglicky

Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvat icus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p&gt;0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex (p&gt;0.05). The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXVI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ

ISBN

9788073059576

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

85-87

Název nakladatele

VETERINÁRNÍ UNIVERZITA BRNO

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2024

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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