Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and sensitivity of PCR and cultivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877904" target="_blank" >RIV/62157124:16270/19:43877904 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00109236

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S037811351830748X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S037811351830748X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetmic.2019.02.004" target="_blank" >10.1016/j.vetmic.2019.02.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and sensitivity of PCR and cultivation

Popis výsledku v původním jazyce

The aim of this study was to determine the seroprevalence of antibodies to B. burgdorferi s.l. in wild small mammals in the Czech Republic and compare sensitivity of PCR and cultivaton. Wild small mammals (n = 691) were trapped in years 2010-2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELISA. Seventy animals were randomly selected for comparison of results of cultivation and PCR. Mean annual antiborelian positivity was 12% with statistical difference (p &lt; 0.05) between Bank Vole (Clethrionomys glareolus) and other six animal species, while there was no statistical difference (p &gt; 0.05) between rodentia and insectivora, gender and localities. The cultivation revealed one positive sample (1.4%), negative in both PCR and ELISA. Method PCR revealed seven positive samples (10%); two of them were simultaneously dubious in ELISA. Eleven animals, negative in cultivation and PCR, had antibodies in ELISA. Method of PCR compared to cultivation seems to be more sensitive for detection of Borrelia.

Název v anglickém jazyce

Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and sensitivity of PCR and cultivation

Popis výsledku anglicky

The aim of this study was to determine the seroprevalence of antibodies to B. burgdorferi s.l. in wild small mammals in the Czech Republic and compare sensitivity of PCR and cultivaton. Wild small mammals (n = 691) were trapped in years 2010-2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELISA. Seventy animals were randomly selected for comparison of results of cultivation and PCR. Mean annual antiborelian positivity was 12% with statistical difference (p &lt; 0.05) between Bank Vole (Clethrionomys glareolus) and other six animal species, while there was no statistical difference (p &gt; 0.05) between rodentia and insectivora, gender and localities. The cultivation revealed one positive sample (1.4%), negative in both PCR and ELISA. Method PCR revealed seven positive samples (10%); two of them were simultaneously dubious in ELISA. Eleven animals, negative in cultivation and PCR, had antibodies in ELISA. Method of PCR compared to cultivation seems to be more sensitive for detection of Borrelia.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

—

Svazek periodika

230

Číslo periodika v rámci svazku

březen

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

241-243

Kód UT WoS článku

000461534600034

EID výsledku v databázi Scopus

2-s2.0-85061659857