Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00143927" target="_blank" >RIV/00216224:14310/25:00143927 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1080/10934529.2026.2613198" target="_blank" >https://doi.org/10.1080/10934529.2026.2613198</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/10934529.2026.2613198" target="_blank" >10.1080/10934529.2026.2613198</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines
Popis výsledku v původním jazyce
The presence of polycyclic aromatic hydrocarbons (PAHs) in ambient air is mainly linked to anthropogenic activities, particularly fossil fuel use and residential wood combustion, posing risks to human health. This study evaluated atmospheric PAHs in Temuco, Chile, characterized their spatial and seasonal distribution, and investigated the in vitro effects of environmentally relevant PAH mixtures on human bronchial epithelial cells (BEAS-2B). Passive air samplers equipped with polyurethane foam (PUF) disks were deployed at two urban sites (Universidad Católica de Temuco and Padre Las Casas) and one rural site (Maquehue sector). Sampling covered summer and fall–early winter, with seasonal assessment conducted in Padre Las Casas. PAHs were quantified by gas chromatography–mass spectrometry (GC–MS), and cell viability was assessed using the MTS assay after exposure to defined PAH mixtures. Spatial variability was observed, with phenanthrene, fluoranthene, and pyrene predominating in urban areas and increasing during winter, while dibenzo(a,h)anthracene was detected exclusively in the rural sector. In BEAS-2B cells, PAH exposure caused a dose and time-dependent reduction in viability, reaching significance at 20 and 28 μM after 48 and 72 h. These findings highlight PAH persistence and potential adverse effects on respiratory epithelial cells, underscoring the need to reduce population exposure.
Název v anglickém jazyce
Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines
Popis výsledku anglicky
The presence of polycyclic aromatic hydrocarbons (PAHs) in ambient air is mainly linked to anthropogenic activities, particularly fossil fuel use and residential wood combustion, posing risks to human health. This study evaluated atmospheric PAHs in Temuco, Chile, characterized their spatial and seasonal distribution, and investigated the in vitro effects of environmentally relevant PAH mixtures on human bronchial epithelial cells (BEAS-2B). Passive air samplers equipped with polyurethane foam (PUF) disks were deployed at two urban sites (Universidad Católica de Temuco and Padre Las Casas) and one rural site (Maquehue sector). Sampling covered summer and fall–early winter, with seasonal assessment conducted in Padre Las Casas. PAHs were quantified by gas chromatography–mass spectrometry (GC–MS), and cell viability was assessed using the MTS assay after exposure to defined PAH mixtures. Spatial variability was observed, with phenanthrene, fluoranthene, and pyrene predominating in urban areas and increasing during winter, while dibenzo(a,h)anthracene was detected exclusively in the rural sector. In BEAS-2B cells, PAH exposure caused a dose and time-dependent reduction in viability, reaching significance at 20 and 28 μM after 48 and 72 h. These findings highlight PAH persistence and potential adverse effects on respiratory epithelial cells, underscoring the need to reduce population exposure.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10511 - Environmental sciences (social aspects to be 5.7)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
ISSN
1093-4529
e-ISSN
1532-4117
Svazek periodika
60
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
633-643
Kód UT WoS článku
001658765600001
EID výsledku v databázi Scopus
2-s2.0-105027979244