Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144628" target="_blank" >RIV/00216224:14310/25:00144628 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsnano.5c13869" target="_blank" >https://pubs.acs.org/doi/10.1021/acsnano.5c13869</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsnano.5c13869" target="_blank" >10.1021/acsnano.5c13869</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles

Popis výsledku v původním jazyce

Upconverting nanoparticles (UCNPs) have emerged as promising alternative donors for resonance energy transfer (FRET)-based biosensing. However, employing UCNPs in FRET assays remains challenging because they display relatively small absorption cross sections and are relatively large as compared to the Forster distance. Thousands of individual donor ions in each UCNP are located within various distances from surface-bound acceptors, complicating the data analysis. While previous studies have explored how the composition and architecture of UCNPs influence FRET, many reports remain qualitative, and multicolor UC-FRET systems involving a single donor and multiple acceptors are less commonly studied than single-donor-single-acceptor systems. To address these challenges, we synthesized UCNPs with an absorbing core (Yb3+-doped)/active shell (Yb3+, Tm3+-doped) nanoparticles systematically varying Tm3+ concentrations to optimize the FRET efficiency to surface-bound organic acceptors. A shell composition containing 4% Tm3+ yielded the highest FRET efficiency. Moreover, four distinct ATTO dyes showing spectral overlap with the blue emission of Tm3+ were used as acceptor dyes on the surface of UCNPs to evaluate FRET efficiencies in spectral and time domains. The differentiation of the four ATTO dyes on one type of upconverting donor nanoparticles using a simple ratiometric approach lays the foundation for the design of multiplexed bioassays. Our results offer a strategy for improving UC-FRET sensitivity through smart core-shell UCNPs designs, donor concentration tuning, and provide important insights into the rational design of more efficient, multicolor, and wash-free UC biosensing platforms.

Název v anglickém jazyce

Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles

Popis výsledku anglicky

Upconverting nanoparticles (UCNPs) have emerged as promising alternative donors for resonance energy transfer (FRET)-based biosensing. However, employing UCNPs in FRET assays remains challenging because they display relatively small absorption cross sections and are relatively large as compared to the Forster distance. Thousands of individual donor ions in each UCNP are located within various distances from surface-bound acceptors, complicating the data analysis. While previous studies have explored how the composition and architecture of UCNPs influence FRET, many reports remain qualitative, and multicolor UC-FRET systems involving a single donor and multiple acceptors are less commonly studied than single-donor-single-acceptor systems. To address these challenges, we synthesized UCNPs with an absorbing core (Yb3+-doped)/active shell (Yb3+, Tm3+-doped) nanoparticles systematically varying Tm3+ concentrations to optimize the FRET efficiency to surface-bound organic acceptors. A shell composition containing 4% Tm3+ yielded the highest FRET efficiency. Moreover, four distinct ATTO dyes showing spectral overlap with the blue emission of Tm3+ were used as acceptor dyes on the surface of UCNPs to evaluate FRET efficiencies in spectral and time domains. The differentiation of the four ATTO dyes on one type of upconverting donor nanoparticles using a simple ratiometric approach lays the foundation for the design of multiplexed bioassays. Our results offer a strategy for improving UC-FRET sensitivity through smart core-shell UCNPs designs, donor concentration tuning, and provide important insights into the rational design of more efficient, multicolor, and wash-free UC biosensing platforms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Projekt

<a href="/cs/project/GF23-06199K" target="_blank" >GF23-06199K: Nové anti-Stokesové lanthanoidové nanočástice a vícebarevný FRET mechanismy pro jednomolekulové sekvenování DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS NANO

ISSN

1936-0851

e-ISSN

1936-086X

Svazek periodika

19

Číslo periodika v rámci svazku

48

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

41110-41120

Kód UT WoS článku

001623202000001

EID výsledku v databázi Scopus

2-s2.0-105024260337