Immunomagnetic separation in the detection of Salmonella cells from foodstuffs

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunomagnetic separation in the detection of Salmonella cells from foodstuffs

Popis výsledku v původním jazyce

Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The abovementioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures.

Název v anglickém jazyce

Immunomagnetic separation in the detection of Salmonella cells from foodstuffs

Popis výsledku anglicky

Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The abovementioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures.

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

13th Internal Symposium of Affinity Technology and Bio-Recognition

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

68

Název nakladatele

Université de Technologie de Compiegne

Místo vydání

Compiegne

Místo konání akce

Compiegne

Datum konání akce

1. 1. 1999

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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