Combined confocal and wide-field high-resolution cytometry of FISH-stained cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F01%3A00004339" target="_blank" >RIV/00216224:14330/01:00004339 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Combined confocal and wide-field high-resolution cytometry of FISH-stained cells

Popis výsledku v původním jazyce

The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-m

Název v anglickém jazyce

Combined confocal and wide-field high-resolution cytometry of FISH-stained cells

Popis výsledku anglicky

The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-m

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytometry

ISSN

0196-4763

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

1

Kód UT WoS článku

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EID výsledku v databázi Scopus

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