Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F02%3A00006215" target="_blank" >RIV/00216224:14330/02:00006215 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/02:17033133

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

Popis výsledku v původním jazyce

Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome.Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nucle

Název v anglickém jazyce

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

Popis výsledku anglicky

Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome.Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nucle

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Micron

ISSN

0968-4328

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

655

Kód UT WoS článku

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EID výsledku v databázi Scopus

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