Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

Popis výsledku v původním jazyce

Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combinehigh-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method forthe preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre

Název v anglickém jazyce

Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

Popis výsledku anglicky

Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combinehigh-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method forthe preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre

Druh

D - Stať ve sborníku

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biophysics of the Genome and Its Interactions

ISBN

80-210-2853-X

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

82

Název nakladatele

Masaryk University

Místo vydání

Brno

Místo konání akce

October 15-17, 2001, Hlohovec, Czech Republic

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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