Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F02%3A00007252" target="_blank" >RIV/00216224:14330/02:00007252 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin

Popis výsledku v původním jazyce

The aim of the therapy of human malignancies is the inhibition of cell proliferation and/or induction of apoptosis. We studied the kinetics of the morphological and biochemical changes in HeLa cells during chemotherapy by cisplatin. Apoptosis was evaluated by scoring of cells exhibiting changes characteristic for early and late stages of apoptosis as determined by Hoehst 33258 staining and by examination of positive reaction for activated caspase-3. Expression and intracellular localization of distinctproteins eas analyzed by immunoblotting of subcellular fractions and segregation of nucleoli by immunocytochemistry.Chromatin fragmentation characteristic for apoptosis was observed in single cells after 3h cisplatin. A strong cytoplasmic accumulation ofcytochrome C detected by immunoblotting 6h post-treatment was accompanied by an activation of caspase-9. Neither inhibition of cell division nor blocking of DNA replication preceded the onset of apoptosis, Our results show that after sho

Název v anglickém jazyce

Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin

Popis výsledku anglicky

The aim of the therapy of human malignancies is the inhibition of cell proliferation and/or induction of apoptosis. We studied the kinetics of the morphological and biochemical changes in HeLa cells during chemotherapy by cisplatin. Apoptosis was evaluated by scoring of cells exhibiting changes characteristic for early and late stages of apoptosis as determined by Hoehst 33258 staining and by examination of positive reaction for activated caspase-3. Expression and intracellular localization of distinctproteins eas analyzed by immunoblotting of subcellular fractions and segregation of nucleoli by immunocytochemistry.Chromatin fragmentation characteristic for apoptosis was observed in single cells after 3h cisplatin. A strong cytoplasmic accumulation ofcytochrome C detected by immunoblotting 6h post-treatment was accompanied by an activation of caspase-9. Neither inhibition of cell division nor blocking of DNA replication preceded the onset of apoptosis, Our results show that after sho

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cancer Detection and Prevention

ISSN

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e-ISSN

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Svazek periodika

accepted

Číslo periodika v rámci svazku

x

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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