Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F06%3A00020052" target="_blank" >RIV/00216224:14330/06:00020052 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells

Popis výsledku v původním jazyce

We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID, which were previously found to be involved in caspase-independent apoptosis. We designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins to study cellular localization and colocalization of these proteins. We predicted the cellular localization of the proteins of interest using software, which analyses the aminoacid sequence of the proteinwith various algorithms. We also designed and applied a novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. Using the listed methods, we managed to confirm by software prediction the localizationof AIF and endoG to mitochondria.

Název v anglickém jazyce

Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells

Popis výsledku anglicky

We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID, which were previously found to be involved in caspase-independent apoptosis. We designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins to study cellular localization and colocalization of these proteins. We predicted the cellular localization of the proteins of interest using software, which analyses the aminoacid sequence of the proteinwith various algorithms. We also designed and applied a novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. Using the listed methods, we managed to confirm by software prediction the localizationof AIF and endoG to mitochondria.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů