Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F07%3A00020613" target="_blank" >RIV/00216224:14330/07:00020613 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells

Popis výsledku v původním jazyce

During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus.

Název v anglickém jazyce

Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells

Popis výsledku anglicky

During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů