Fluorescent microscopy of living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F07%3A00020659" target="_blank" >RIV/00216224:14330/07:00020659 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescent microscopy of living cells

Popis výsledku v původním jazyce

Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achievetwo- or three-color imaging.

Název v anglickém jazyce

Fluorescent microscopy of living cells

Popis výsledku anglicky

Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achievetwo- or three-color imaging.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů