Optimizing light throughput in image cytometry
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F07%3A00023729" target="_blank" >RIV/00216224:14330/07:00023729 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Optimizing light throughput in image cytometry
Popis výsledku v původním jazyce
Image cytometry applications often suffer from the lack of light due to the use of fluorescence mode. Low light levels typically cause poor signal-to-noise ratio or long exposure times. Signal level in fluorescence set-up is determined by many factors, such as power of the light source and its spectral characteristics, transmission curves of excitation/emission filters, transmission curves of and dichroic/polychroic mirrors, excitation/emission spectra of imaged fluorochrome(s), numerical aperture of the objective and its transmission curve, quantum efficiency curve of the light detector. Thus, resulting light throughput is given by the composition of spectral characteristics (functions of wavelength) of many components. Proper computation and optimization of light throughput is a non-trivial task that requires knowledge of all these curves for all available hardware components as well as all available fluorochromes.
Název v anglickém jazyce
Optimizing light throughput in image cytometry
Popis výsledku anglicky
Image cytometry applications often suffer from the lack of light due to the use of fluorescence mode. Low light levels typically cause poor signal-to-noise ratio or long exposure times. Signal level in fluorescence set-up is determined by many factors, such as power of the light source and its spectral characteristics, transmission curves of excitation/emission filters, transmission curves of and dichroic/polychroic mirrors, excitation/emission spectra of imaged fluorochrome(s), numerical aperture of the objective and its transmission curve, quantum efficiency curve of the light detector. Thus, resulting light throughput is given by the composition of spectral characteristics (functions of wavelength) of many components. Proper computation and optimization of light throughput is a non-trivial task that requires knowledge of all these curves for all available hardware components as well as all available fluorochromes.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
JD - Využití počítačů, robotika a její aplikace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC535" target="_blank" >LC535: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2007
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů