Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating - Analysis of high-temperature fluorescence rise around 60 degrees C

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F00%3A00004746" target="_blank" >RIV/60076658:12640/00:00004746 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating - Analysis of high-temperature fluorescence rise around 60 degrees C

Popis výsledku v původním jazyce

The spectral characteristics of chlorophyll fluorescence and absorption during linear heating of barley leaves within the range 25-75 degreesC (fluorescence temperature curve, FTC) were studied. Leaves with various content of light harvesting complexes (green, Chl b-less chlorina f2 and intermittent light grown) revealing different types of FTC were used. Differential 'absorption, emission and excitation spectra documented four characteristic phases of the FTC. The initial two FTC phases (a rise in the46-49 degreesC region and a subsequent decrease to about 55 degreesC) mostly reflected changes in the fluorescence quantum yield peaking at about 685 nm. A steep second fluorescence rise at 55-61 degreesC was found to originate from a short-wavelength Chl a spectral form (emission maximum at 675 nm) causing a gradual blue shift of the emission spectra. In this temperature range, a clear correspondence of the blue shift in the emission and absorption spectra was found. We suggest that the

Název v anglickém jazyce

Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating - Analysis of high-temperature fluorescence rise around 60 degrees C

Popis výsledku anglicky

The spectral characteristics of chlorophyll fluorescence and absorption during linear heating of barley leaves within the range 25-75 degreesC (fluorescence temperature curve, FTC) were studied. Leaves with various content of light harvesting complexes (green, Chl b-less chlorina f2 and intermittent light grown) revealing different types of FTC were used. Differential 'absorption, emission and excitation spectra documented four characteristic phases of the FTC. The initial two FTC phases (a rise in the46-49 degreesC region and a subsequent decrease to about 55 degreesC) mostly reflected changes in the fluorescence quantum yield peaking at about 685 nm. A steep second fluorescence rise at 55-61 degreesC was found to originate from a short-wavelength Chl a spectral form (emission maximum at 675 nm) causing a gradual blue shift of the emission spectra. In this temperature range, a clear correspondence of the blue shift in the emission and absorption spectra was found. We suggest that the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/VS96085" target="_blank" >VS96085: Studium vztahů mezi funkcí biologických membrán a interakcemi jejich složek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Photochemistry and Photobiology B-Biology

ISSN

1011-1344

e-ISSN

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Svazek periodika

59

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

103-114

Kód UT WoS článku

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EID výsledku v databázi Scopus

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