cDNA Microarray: Expresní profilování malých buněčných pupulací

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F08%3A00027029" target="_blank" >RIV/00216224:14330/08:00027029 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

cDNA Microarray: Gene Expression Profiling of Small Cell Populations

Popis výsledku v původním jazyce

cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small populationin bone marrow (1- 4% of all cells). Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for linear

Název v anglickém jazyce

cDNA Microarray: Gene Expression Profiling of Small Cell Populations

Popis výsledku anglicky

cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small populationin bone marrow (1- 4% of all cells). Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for linear

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů