Prediction of localization and interactions of proteins involved in caspase-independent apoptosis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F09%3A00036811" target="_blank" >RIV/00216224:14330/09:00036811 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Prediction of localization and interactions of proteins involved in caspase-independent apoptosis
Popis výsledku v původním jazyce
During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and endonuclease G (endoG). Another interesting protein is AIF-homologous mitochondrion-associated inducer of death (AMID). Heat shock protein HSP70-1, cyclophilin A and possibly DNA topoisomerase II alpha are also high candidate proteins that seem to take part at least in some part of caspase-independent apoptosis connected toAIF. We studied the structure, cellular localization, and interactions of these proteins in silico and also in cells using fluorescent microscopy. Bioinformatic predictions were conducted to analyze the interactions of some of the studied proteins with possible partners. We conducted molecular modeling of proteins with unknown 3D structures. These models were then refined by MolProbity server and employed in molecular docking simulations of interactions.
Název v anglickém jazyce
Prediction of localization and interactions of proteins involved in caspase-independent apoptosis
Popis výsledku anglicky
During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and endonuclease G (endoG). Another interesting protein is AIF-homologous mitochondrion-associated inducer of death (AMID). Heat shock protein HSP70-1, cyclophilin A and possibly DNA topoisomerase II alpha are also high candidate proteins that seem to take part at least in some part of caspase-independent apoptosis connected toAIF. We studied the structure, cellular localization, and interactions of these proteins in silico and also in cells using fluorescent microscopy. Bioinformatic predictions were conducted to analyze the interactions of some of the studied proteins with possible partners. We conducted molecular modeling of proteins with unknown 3D structures. These models were then refined by MolProbity server and employed in molecular docking simulations of interactions.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů