Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F11%3A00053487" target="_blank" >RIV/00216224:14330/11:00053487 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells

Popis výsledku v původním jazyce

Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria, then they translocate intothe cell nuclei, where they participate in chromatin degradation in a caspase-independent way. We have conducted living-cell confocal fluorescence microscopy followed by analysis of fluorescence resonance energy transfer (FRET) to observe the protein interaction of EndoG with AIF and their interactions with other proteins in human cell nuclei after induction of apoptosis. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interactwith TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus.

Název v anglickém jazyce

Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells

Popis výsledku anglicky

Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria, then they translocate intothe cell nuclei, where they participate in chromatin degradation in a caspase-independent way. We have conducted living-cell confocal fluorescence microscopy followed by analysis of fluorescence resonance energy transfer (FRET) to observe the protein interaction of EndoG with AIF and their interactions with other proteins in human cell nuclei after induction of apoptosis. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interactwith TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů