HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F14%3A00074061" target="_blank" >RIV/00216224:14330/14:00074061 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >http://dx.doi.org/10.1186/1756-8935-7-39</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >10.1186/1756-8935-7-39</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Popis výsledku v původním jazyce

Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.

Název v anglickém jazyce

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Popis výsledku anglicky

Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Epigenetics & Chromatin

ISSN

1756-8935

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

39

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

000347931500001

EID výsledku v databázi Scopus

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