HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00441300" target="_blank" >RIV/68081707:_____/14:00441300 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/14:10285734

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >http://dx.doi.org/10.1186/1756-8935-7-39</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >10.1186/1756-8935-7-39</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Popis výsledku v původním jazyce

Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.

Název v anglickém jazyce

HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Popis výsledku anglicky

Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Epigenetics & Chromatin

ISSN

1756-8935

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

DEC2014

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

000347931500001

EID výsledku v databázi Scopus

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