THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14410%2F07%3A00032805" target="_blank" >RIV/00216224:14410/07:00032805 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
Popis výsledku v původním jazyce
Yarrowia lipolytica has two mating types designated A and B. Mating type A cells produce mating specific pheromone (A factor). Crude fractions of the A factor was partially purified and concentrated (1.000 fold) by Amberlite CG 50. This A factor acts only on cells of opposite mating type inducning a cascade of metabolic and morphological changes. Ths A factor specially inhibits the cell division of mating type B. The cell were arrested in the G1 phase (no budding, characterized by light microscopy). Thearrest is reversible and the lenght of the arrest period is dependent on the concentration of A factor added. The A factor is one element of a relatory system whose functions assure the synchronization of haploid cells for conjugation. In an asynchronous population only a small part of cells are in this specific stage. When A factor is added to conjugation medium (YM), the time for conjugation was shorter and the conjugation rate was increased.
Název v anglickém jazyce
THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
Popis výsledku anglicky
Yarrowia lipolytica has two mating types designated A and B. Mating type A cells produce mating specific pheromone (A factor). Crude fractions of the A factor was partially purified and concentrated (1.000 fold) by Amberlite CG 50. This A factor acts only on cells of opposite mating type inducning a cascade of metabolic and morphological changes. Ths A factor specially inhibits the cell division of mating type B. The cell were arrested in the G1 phase (no budding, characterized by light microscopy). Thearrest is reversible and the lenght of the arrest period is dependent on the concentration of A factor added. The A factor is one element of a relatory system whose functions assure the synchronization of haploid cells for conjugation. In an asynchronous population only a small part of cells are in this specific stage. When A factor is added to conjugation medium (YM), the time for conjugation was shorter and the conjugation rate was increased.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2007
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů