Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14610%2F19%3A00111213" target="_blank" >RIV/00216224:14610/19:00111213 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1107/S2059798319011860" target="_blank" >http://dx.doi.org/10.1107/S2059798319011860</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S2059798319011860" target="_blank" >10.1107/S2059798319011860</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion

Popis výsledku v původním jazyce

Electron microscopy of macromolecular structures is an approach that is in increasing demand in the field of structural biology. The automation of image acquisition has greatly increased the potential throughput of electron microscopy. Here, the focus is on the possibilities in Scipion to implement flexible and robust image-processing workflows that allow the electron-microscope operator and the user to monitor the quality of image acquisition, assessing very simple acquisition measures or obtaining a first estimate of the initial volume, or the data resolution and heterogeneity, without any need for programming skills. These workflows can implement intelligent automatic decisions and they can warn the user of possible acquisition failures. These concepts are illustrated by analysis of the well known 2.2 A resolution beta-galactosidase data set.

Název v anglickém jazyce

Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion

Popis výsledku anglicky

Electron microscopy of macromolecular structures is an approach that is in increasing demand in the field of structural biology. The automation of image acquisition has greatly increased the potential throughput of electron microscopy. Here, the focus is on the possibilities in Scipion to implement flexible and robust image-processing workflows that allow the electron-microscope operator and the user to monitor the quality of image acquisition, assessing very simple acquisition measures or obtaining a first estimate of the initial volume, or the data resolution and heterogeneity, without any need for programming skills. These workflows can implement intelligent automatic decisions and they can warn the user of possible acquisition failures. These concepts are illustrated by analysis of the well known 2.2 A resolution beta-galactosidase data set.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Crystallographica Section D

ISSN

2059-7983

e-ISSN

—

Svazek periodika

75

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

882-894

Kód UT WoS článku

000497657100003

EID výsledku v databázi Scopus

2-s2.0-85072916357