FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14610%2F20%3A00116269" target="_blank" >RIV/00216224:14610/20:00116269 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2079-9292/9/6/1040" target="_blank" >https://www.mdpi.com/2079-9292/9/6/1040</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/electronics9061040" target="_blank" >10.3390/electronics9061040</a>

Jazyk výsledku

angličtina

Název v původním jazyce

FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Popis výsledku v původním jazyce

Cryogenic Electron Microscopy (Cryo-EM) has been established as one of the key players in Structural Biology. It can reconstruct a 3D model of the sample at the near-atomic resolution, which led to a Method of the year award by Nature, and the Nobel Prize in 2017. With the growing number of facilities, faster microscopes, and new imaging techniques, new algorithms are needed to process the so-called movies data produced by the microscopes in real-time, while preserving a high resolution and maximum of additional information. In this article, we present a new algorithm used for movie alignment, called FlexAlign. FlexAlign is able to correctly compensate for the shift produced during the movie acquisition on-the-fly, using the current generation of hardware. The algorithm performs a global and elastic local registration of the movie frames using Cross-Correlation and B-spline interpolation for high precision. We show that our execution time is compatible with real-time correction and that we preserve the high-resolution information up to high frequency.

Název v anglickém jazyce

FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Popis výsledku anglicky

Cryogenic Electron Microscopy (Cryo-EM) has been established as one of the key players in Structural Biology. It can reconstruct a 3D model of the sample at the near-atomic resolution, which led to a Method of the year award by Nature, and the Nobel Prize in 2017. With the growing number of facilities, faster microscopes, and new imaging techniques, new algorithms are needed to process the so-called movies data produced by the microscopes in real-time, while preserving a high resolution and maximum of additional information. In this article, we present a new algorithm used for movie alignment, called FlexAlign. FlexAlign is able to correctly compensate for the shift produced during the movie acquisition on-the-fly, using the current generation of hardware. The algorithm performs a global and elastic local registration of the movie frames using Cross-Correlation and B-spline interpolation for high precision. We show that our execution time is compatible with real-time correction and that we preserve the high-resolution information up to high frequency.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

<a href="/cs/project/EF16_013%2F0001802" target="_blank" >EF16_013/0001802: CERIT Scientific Cloud</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electronics

ISSN

2079-9292

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

25

Strana od-do

1-25

Kód UT WoS článku

000551113800001

EID výsledku v databázi Scopus

2-s2.0-85086840250