5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00049942" target="_blank" >RIV/00216224:14740/11:00049942 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/11:00367828

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10858-011-9496-2" target="_blank" >http://dx.doi.org/10.1007/s10858-011-9496-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10858-011-9496-2" target="_blank" >10.1007/s10858-011-9496-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

Popis výsledku v původním jazyce

Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguousassignment of the disordered part of the protein from a single 5D spectrum.

Název v anglickém jazyce

5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

Popis výsledku anglicky

Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguousassignment of the disordered part of the protein from a single 5D spectrum.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomolecular NMR

ISSN

0925-2738

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

000290044400001

EID výsledku v databázi Scopus

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